Rivers Laboratory

Abstracts

Anne Gallivan, Amarachi Kanu, Mikail Alejandro, Nebeyat Zekaryas, Hart Horneman, Jagadeesh Ramasamy, Lenny Hong, Donald Lavelle, Alan Diamond, Robert E. Molokie, Elliott P. Vichinsky, Angela Rivers; Reticulocytosis from Stress Erythropoiesis Is a Major Source of Erythrocyte Mitochondrial Retention, Oxygen Consumption and Reactive Oxygen Species in a SCD Mouse Model. Blood 2022; 140 (Supplement 1): 8239–8240. doi: https://doi.org/10.1182/blood-2022-160481
Jagadeesh Ramasamy, Vinzon Ibanez, Vijaya Lakshmi Yalagala, Yogenthiran Saunthararajah, Robert E. Molokie, Angela Rivers, Donald Lavelle; Nicotinamide Improves Anemia and Decreases Reactive Oxygen Species in Sickle Cell Disease Mice. Blood 2021; 138 (Supplement 1): 2019. doi: https://doi.org/10.1182/blood-2021-144956
Jagadeesh Ramasamy, Vinzon Ibanez, Kareem Al-Qadi, Helen Zhang, Jennifer Afranie-Sakyi, Robert E. Molokie, Donald Lavelle, Angela Rivers; Cathepsin B, a Negative Regulator of Autophagy, Identified As a Novel Therapeutic Drug Target in Sickle Cell Disease. Blood 2020; 136 (Supplement 1): 32. doi: https://doi.org/10.1182/blood-2020-140119
Ramasamy Jagadeeswaran, Hong Lenny, Benjamin Vazquez, Joshua Muniz, Aimee Schad, Shivi Jain, Michel Gowhari, Donald Lavelle, Alan Diamond, Robert E. Molokie, Angela Rivers; The Abnormal Presence of Mitochondria in Circulating Red Blood Cells Cause an Increased Oxygen Consumption Rate, ROS Generation and Hemolysis in Patients with Sickle Cell Disease. Blood 2017; 130 (Supplement 1): 2237. doi: https://doi.org/10.1182/blood.V130.Suppl_1.2237.2237

Life Science Publications

Gallivan, A., Alejandro, M., Kanu, A., Zekaryas, N., Horneman, H., Hong, L. K., Vinchinsky, E., Lavelle, D., Diamond, A. M., Molokie, R. E., Ramasamy, J., & Rivers, A. (2023). Reticulocyte mitochondrial retention increases reactive oxygen species and oxygen consumption in mouse models of sickle cell disease and phlebotomy-induced anemia. Experimental Hematology. https://doi.org/10.1016/j.exphem.2023.02.005
Rivers A, Vaitkus K, Jagadeeswaran R, Ruiz MA, Ibanez V, Ciceri F, Cavalcanti F, Molokie RE, Saunthararajah Y, Engel JD, DeSimone J, Lavelle D. Oral administration of the LSD1 inhibitor ORY-3001 increases fetal hemoglobin in sickle cell mice and baboons. Exp Hematol. 2018 Nov;67:60-64.e2. doi: 10.1016/j.exphem.2018.08.003. Epub 2018 Aug 17. PubMed PMID: 30125603; PubMed Central PMCID: PMC8728698.
Jagadeeswaran R, Vazquez BA, Thiruppathi M, Ganesh BB, Ibanez V, Cui S, Engel JD, Diamond AM, Molokie RE, DeSimone J, Lavelle D, Rivers A. Pharmacological inhibition of LSD1 and mTOR reduces mitochondrial retention and associated ROS levels in the red blood cells of sickle cell disease. Exp Hematol. 2017 Jun;50:46-52. doi: 10.1016/j.exphem.2017.02.003. Epub 2017 Feb 24. PubMed PMID: 28238805; PubMed Central PMCID: PMC6263958.
Ibanez V, Vaitkus K, Rivers A, Molokie R, Cui S, Engel JD, DeSimone J, Lavelle D. Efficacy and safety of long-term RN-1 treatment to increase HbF in baboons. Blood. 2017 Jan 12;129(2):260-263. doi: 10.1182/blood-2016-10-746727. Epub 2016 Dec 1. PubMed PMID: 27908882; PubMed Central PMCID: PMC5234223.
Rivers A, Vaitkus K, Ibanez V, Ruiz MA, Jagadeeswaran R, Saunthararajah Y, Cui S, Engel JD, DeSimone J, Lavelle D. The LSD1 inhibitor RN-1 recapitulates the fetal pattern of hemoglobin synthesis in baboons (P. anubis). Haematologica. 2016 Jun;101(6):688-97. doi: 10.3324/haematol.2015.140749. Epub 2016 Feb 8. PubMed PMID: 26858356; PubMed Central PMCID: PMC5013966.
Cui S, Lim KC, Shi L, Lee M, Jearawiriyapaisarn N, Myers G, Campbell A, Harro D, Iwase S, Trievel RC, Rivers A, DeSimone J, Lavelle D, Saunthararajah Y, Engel JD. The LSD1 inhibitor RN-1 induces fetal hemoglobin synthesis and reduces disease pathology in sickle cell mice. Blood. 2015 Jul 16;126(3):386-96. doi: 10.1182/blood-2015-02-626259. Epub 2015 Jun 1. PubMed PMID: 26031919; PubMed Central PMCID: PMC4504950.
Rivers A, Vaitkus K, Ruiz MA, Ibanez V, Jagadeeswaran R, Kouznetsova T, DeSimone J, Lavelle D. RN-1, a potent and selective lysine-specific demethylase 1 inhibitor, increases γ-globin expression, F reticulocytes, and F cells in a sickle cell disease mouse model. Exp Hematol. 2015 Jul;43(7):546-53.e1-3. doi: 10.1016/j.exphem.2015.04.005. Epub 2015 Apr 27. PubMed PMID: 25931013; PubMed Central PMCID: PMC6325640.
Ruiz MA, Rivers A, Ibanez V, Vaitkus K, Mahmud N, DeSimone J, Lavelle D. Hydroxymethylcytosine and demethylation of the γ-globin gene promoter during erythroid differentiation. Epigenetics. 2015;10(5):397-407. doi: 10.1080/15592294.2015.1039220. PubMed PMID: 25932923; PubMed Central PMCID: PMC4622718.
Pandya J, Ortiz L, Ling C, Rivers AE, Aslanidi G. Rationally designed capsid and transgene cassette of AAV6 vectors for dendritic cell-based cancer immunotherapy. Immunol Cell Biol. 2014 Feb;92(2):116-23. doi: 10.1038/icb.2013.74. Epub 2013 Nov 12. PubMed PMID: 24217810.
Choe SW, Terman DS, Rivers AE, Rivera J, Lottenberg R, Sorg BS. Drug-loaded sickle cells programmed ex vivo for delayed hemolysis target hypoxic tumor microvessels and augment tumor drug delivery. J Control Release. 2013 Oct 28;171(2):184-92. doi: 10.1016/j.jconrel.2013.07.008. Epub 2013 Jul 18. PubMed PMID: 23871960; PubMed Central PMCID: PMC4162096.
Song L, Kauss MA, Kopin E, Chandra M, Ul-Hasan T, Miller E, Jayandharan GR, Rivers AE, Aslanidi GV, Ling C, Li B, Ma W, Li X, Andino LM, Zhong L, Tarantal AF, Yoder MC, Wong KK Jr, Tan M, Chatterjee S, Srivastava A. Optimizing the transduction efficiency of capsid-modified AAV6 serotype vectors in primary human hematopoietic stem cells in vitro and in a xenograft mouse model in vivo. Cytotherapy. 2013 Aug;15(8):986-98. doi: 10.1016/j.jcyt.2013.04.003. PubMed PMID: 23830234; PubMed Central PMCID: PMC3711144.
Aslanidi GV, Rivers AE, Ortiz L, Song L, Ling C, Govindasamy L, Van Vliet K, Tan M, Agbandje-McKenna M, Srivastava A. Optimization of the capsid of recombinant adeno-associated virus 2 (AAV2) vectors: the final threshold?. PLoS One. 2013;8(3):e59142. doi: 10.1371/journal.pone.0059142. Epub 2013 Mar 19. PubMed PMID: 23527116; PubMed Central PMCID: PMC3602601.
Aslanidi GV, Rivers AE, Ortiz L, Govindasamy L, Ling C, Jayandharan GR, Zolotukhin S, Agbandje-McKenna M, Srivastava A. High-efficiency transduction of human monocyte-derived dendritic cells by capsid-modified recombinant AAV2 vectors. Vaccine. 2012 Jun 6;30(26):3908-17. doi: 10.1016/j.vaccine.2012.03.079. Epub 2012 Apr 10. PubMed PMID: 22497875; PubMed Central PMCID: PMC3356484.
Jayandharan GR, Zhong L, Sack BK, Rivers AE, Li M, Li B, Herzog RW, Srivastava A. Optimized adeno-associated virus (AAV)-protein phosphatase-5 helper viruses for efficient liver transduction by single-stranded AAV vectors: therapeutic expression of factor IX at reduced vector doses. Hum Gene Ther. 2010 Mar;21(3):271-83. doi: 10.1089/hum.2009.100. PubMed PMID: 19788390; PubMed Central PMCID: PMC2865358.

Review Article Publications

Campbell AD, Colombatti R, Andemariam B, Strunk C, Tartaglione I, Piccone CM, Manwani D, Asare EV, Boruchov D, Farooq F, Urbonya R, Boatemaa GD, Perrotta S, Sainati L, Rivers A, Rao S, Zempsky W, Sey F, Segbefia C, Inusa B, Antwi-Boasiako C. An Analysis of Racial and Ethnic Backgrounds Within the CASiRe International Cohort of Sickle Cell Disease Patients: Implications for Disease Phenotype and Clinical Research. J Racial Ethn Health Disparities. 2021 Feb;8(1):99-106. doi: 10.1007/s40615-020-00762-2. Epub 2020 May 16. PubMed PMID: 32418182; PubMed Central PMCID: PMC7669607.
Mittal N, Loughran T Jr, Rivers A. T-cell large granular lymphocytic leukemia successfully treated with oral cyclosporine in a child: Case report and review of literature. Pediatr Blood Cancer. 2020 Dec;67(12):e28487. doi: 10.1002/pbc.28487. Epub 2020 Jun 26. Review. PubMed PMID: 32589344.
Antwi-Boasiako C, Andemariam B, Colombatti R, Asare EV, Strunk C, Piccone CM, Manwani D, Boruchov D, Farooq F, Urbonya R, Wilson S, Boatemaa GD, Perrotta S, Sainati L, Rivers A, Rao S, Zempsky W, Ekem I, Sey F, Segbefia C, Inusa B, Tartaglione I, Campbell AD. A study of the geographic distribution and associated risk factors of leg ulcers within an international cohort of sickle cell disease patients: the CASiRe group analysis. Ann Hematol. 2020 Sep;99(9):2073-2079. doi: 10.1007/s00277-020-04057-8. Epub 2020 May 6. PubMed PMID: 32377816; PubMed Central PMCID: PMC7430152.
Rivers A. Cognition affected in young children with serum ferritin <17 μg/L. J Pediatr. 2020 May;220:264-267. doi: 10.1016/j.jpeds.2020.02.065. PubMed PMID: 32334667.
Rivers A, Molokie R, Lavelle D. A new target for fetal hemoglobin reactivation. Haematologica. 2019 Dec;104(12):2325-2327. doi: 10.3324/haematol.2019.230904. PubMed PMID: 31787612; PubMed Central PMCID: PMC6959183.
Rivers A, Jagadeeswaran R, Lavelle D. Potential role of LSD1 inhibitors in the treatment of sickle cell disease: a review of preclinical animal model data. Am J Physiol Regul Integr Comp Physiol. 2018 Oct 1;315(4):R840-R847. doi: 10.1152/ajpregu.00440.2017. Epub 2018 Aug 1. Review. PubMed PMID: 30067082; PubMed Central PMCID: PMC6734057.
Jagadeeswaran R, Rivers A. Evolving treatment paradigms in sickle cell disease. Hematology Am Soc Hematol Educ Program. 2017 Dec 8;2017(1):440-446. doi: 10.1182/asheducation-2017.1.440. Review. PubMed PMID: 29222291; PubMed Central PMCID: PMC6142561.
Han J, Kemiki O, Hsu LL, Rivers AE. Adverse Reactions to Pneumococcal Vaccine in Pediatric and Adolescent Patients with Sickle Cell Disease. Pharmacotherapy. 2015 Jul;35(7):696-700. doi: 10.1002/phar.1607. Epub 2015 Jun 19. PubMed PMID: 26095207; PubMed Central PMCID: PMC5520624.
Rivers A, Slayton WB. Congenital cytopenias and bone marrow failure syndromes. Semin Perinatol. 2009 Feb;33(1):20-8. doi: 10.1053/j.semperi.2008.10.009. Review. PubMed PMID: 19167578.
Slayton WB, Patel M, Sola-Visner M, Harris N, Rivers A, Montgomery RR, Friedman KD. Type 2B von Willebrand disease associated with the release of platelet agglutinates from megakaryocytes in the bone marrow. J Pediatr Hematol Oncol. 2008 Sep;30(9):708-11. doi: 10.1097/MPH.0b013e31817541cd. PubMed PMID: 18776767; PubMed Central PMCID: PMC5592795.
Maina N, Zhong L, Li X, Zhao W, Han Z, Bischof D, Aslanidi G, Zolotukhin S, Weigel-Van Aken KA, Rivers AE, Slayton WB, Yoder MC, Srivastava A. Optimization of recombinant adeno-associated viral vectors for human beta-globin gene transfer and transgene expression. Hum Gene Ther. 2008 Apr;19(4):365-75. doi: 10.1089/hum.2007.173. PubMed PMID: 18399730.